ABSTRACT
OBJECTIVES@#Stably expressed transforming growth factor -beta 1(TGF-β1)MCs were obtained and the effects of centellaasiatica (CA) granule on the expressions of Smad 2/3, Smad 7 and collagen Ⅳ and the level of Smad 2/3 phosphorylation were observed.@*METHODS@#Lipofectin method was used to transfect TGF-β1 vector into MC, and the stably expressed TGF-β1 cell lines were selected by G418. The cells were divided into three groups. Control group:normal MC + RPMI 1640 + 10% normal rat serum; TGF-β1 group:stably expressed TGF-β1 MC + RPMI 1640 + 10% normal rat serum; CA group:stably expressed TGF-β1 MC + RPMI 1640 + 10% rat serum containing high CA. The experiments were repeated for five times. The contents of TGF-β1 and collagen Ⅳ in the culture medium were detected with ELISA, the expressions of mRNA and protein of TGF-β1, Smad 2/3, Smad 7 and the level of Smad 2/3 phosphorylation were detected by using real time quantitative polymerase chain reaction and Western blot.@*RESULTS@#The contents of TGF-β1 and collagen Ⅳ in the culture medium of stably-expressed TGF-β1 MC were increased significantly, and the CA could reverse the effects of TGF-β1. The expressions of mRNA and protein of TGF-β1, Smad 2/3 and the level of Smad 2/3 phosphorylation were increased significantly in TGF-β1 transfected MC, and CA could dramatically reduce the expressions of mRNA and protein of TGF-β1, Smad 2/3 and the level of Smad 2/3 phosphorylation. The high expression of TGF-β1 decreased the expression of Smad 7 mRNA and protein, and the CA could antagonize the effect of mRNA expression.@*CONCLUSIONS@#The MCs stably-expressed TGF-β1 can activate the TGF-β1/Smad signal pathway and increase the expression of collagen Ⅳ. CA can decrease the occurrence of diabetic nephropathy(DN) by reducing the production of collagen Ⅳ through inhibiting the TGF-β1/Smad signal pathway.
Subject(s)
Animals , Rats , Cells, Cultured , Centella , Chemistry , Collagen Type IV , Metabolism , Drugs, Chinese Herbal , Pharmacology , Mesangial Cells , Metabolism , Signal Transduction , Smad Proteins , Metabolism , Smad2 Protein , Metabolism , Smad3 Protein , Metabolism , Smad7 Protein , Metabolism , Transforming Growth Factor beta1 , MetabolismABSTRACT
·AIM: To investigate the distribution of serum specific IgE in children with allergic conjunctivitis and associated allergic diseases. ·METHODS: Retrospective analysis of 708 cases of allergic conjunctivitis in children, according to age divided into the infant group (2 months to 1 years old) 232 cases, the children group (>1 years to 3 years old) 255 cases and the preschool group (>3 years to 6 years old) 221 cases. A automatic in vitro detection system was used to detect serum inhaled allergens and food allergen specific IgE by immune capture method. A questionnaire survey was conducted to investigate the associated allergic diseases and consultation with relevant departments. ·RESULTS: The sIgE positive rate was the lowest in the infant group (87.1%). There were significant differences in the number of sIgE positive species in the infant group compared with those in the other two groups (χ2=10. 96, 21. 78; P<0. 01). The most common allergens in all three groups were milk, egg white and household dust mites, and the positive rate of SIgE in milk was higher in the infant group than in the other two groups. The positive rate of sIgE in dust mites, house dust, tree pollen, mulberry, dog fur, egg white, pineapple and mango were higher in the preschool group than in the other two groups. The positive rate of 3-6 grade sIgE in household dust mites and house dust were higher in preschool group than that in the other two groups (P<0. 01). The infant group had the highest proportion of gastrointestinal allergy (28. 9%). The preschool group had the highest proportion of allergic rhinitis. The proportion with more than three kinds of allergic diseases in children group was higher than that of the other two groups(P<0. 01). ·CONCLUSION: With the increase of age, the positive rate and types of allergen in children with allergic conjunctivitis increased gradually. House dust mites become the primary inhalation allergen from infancy. Allergic diseases associated with allergic conjunctivitis in children are consistent with allergic march.
ABSTRACT
Objective To analyze changes of three periodical circulation systems,erythrocyte sedimentation rate and bone marrow cell morphology in children with malaria.Methods The routine tests of hematology by Sysmex KX-21 Counter, erythrocyte sedimentation rate by Westergren method and bone marrow cell morphology were analyzed. Results In 22 cases of malaria the ratio of Hb level below 110 g/L,WBC below 4?10~9/L and PLT below 100?10~9/L was 68.2%, 41.0%, and 77.3%,respectively. The ratio of children with all three parameters (Hb, WBC and PLT) abnormal was 36.4%, with two parameters abnormal was 63.6%. Ninty-five point five percent of malaria children′s erythrocyte sedimentation rate was abnormal. Fifty-nine point one percent of malaria children had hyperplasia anemia bone marrow morphology, 77.3% secondary thrombocytopenia and 54.5% with both of two bone marrow morphology.Conclusions Three periodical circulation systems of malaria children alter notably, especially in PLT and Hb. The majority has erythrocyte sedimentation rate abnormal, and bone marrow cell morphology shows hyperplasia anemia and thrombocytopenia.